James G. Fujimoto and Daniel Farkas
Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and
applications.
A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The
integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction.
Novel techniques integrate microscopy with state-of-the-art optics technology, and these
include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and
light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables
molecular specific sensitivity.
James G. Fujimoto and Daniel L. Farkas: Preface
1. Tony Wilson: Confocal Microscopy
2. Kevin Burton, Jihoon Jeong, Sebastian Wachsmann-Hogiu and Daniel L. Farkas: Spectral Optical Imaging in Biology and Medicine
3. Fritjof Helmchen, Samuel S.-H. Wang, and Winifred Denk:
Multi-photon Microscopy in Neuroscience
4. Dahlene Fusco, Edouard Bertrand, and Robert H. Singer: mRNA Imaging in Living Cells for Biomedical Research
5. Alan S. Waggoner, Lauren A. Ernst, and Byron Ballou: Building New Fluorescent Probes
6. Dejan Vucinic, Efstratios Kosmidis, Chun X.
Falk, Lawrence B. Cohen, Leslie M. Loew, Maja Djurisic, and Dejan Zecevic: Imaging Membrane Potential with Voltage-Sensitive Dyes
7. James Fujimoto, Yu Chen, and Aaron Aguirre: Biomedical Imaging using Optical Coherence Tomography
8. Petra Schwille, Katrin Heinze, Petra Dittrich, and Elke
Haustein: Two-Photon Fluorescence Correlation Spectroscopy
9. Stefan W. Hell and Andreas Schönle: Nanoscopy: The Future of Optical Microscopy
10. S. Andersson-Engels, K. Svanberg, and S. Svanberg: Fluorescence Imaging in Medical Diagnostics
11. Adam Wax, Vadim Backman, Changhuei Yang,
and Michael S. Feld: Light Scattering Spectroscopic Techniques for Examining Cellular Structure, Organization and Dynamics
12. I. Pavlova, R. Drezek, S. Chang, D. Arifler, K. Sokolov, C. MacAulay, M. Follen, and R. Richards-Kortum: Fluorescence and Spectroscopic Markers of Cervical Neoplasia
13. Dorota Jakubowski, Frederic Bevilacqua, Sean Merritt, Albert Cerussi, and Bruce J. Tromberg: Quantitative Absorption and Scattering Spectra in Thick Tissues using Broadband Diffuse Optical Spectroscopy
14. Enrico Gratton, Vlad Toronov, Ursula Wolf, and Martin Wolf: Detection of Brain
Activity by Near-Infrared Light
15. Vasilis Ntziachristos and Ralph Weissleder: In-Vivo Optical Imaging of Molecular Function using NIR Fluorescent Probes
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James G. Fujimoto is Professor of Electrical Engineering and Computer Science at the Massachusetts Institute of Technology, where he works on the development and application of femtosecond laser technology and studies ultrafast phenomena and biomedical optics. He received his bachelors,
masters, and doctorate degrees from M.I.T.
Daniel Farkas is Vice Chairman for Research in the Department of Surgery and Director of the Minimally Invasive Surgical Technologies Institute at the Cedars-Sinai Medical Center in Los Angeles. He is also a Research Professor in Biomedical
Engineering at the University of Southern California, and Adjunct Professor at the Robotics Institute at Carnegie Mellon University. Farkas was trained in theoretical physics in Romania, and holds a Ph.D. in Biophysics and Biochemistry from the Weizmann Institute in Israel.